P090 Low-density neutrophils are higher in individuals with active ulcerative colitis

Abstract Background Introduction: Neutrophils and neutrophil extracellular traps (NETs) play a significant role in the pathogenesis of ulcerative colitis (UC), chronic relapsing inflammatory disease colon. Low-density neutrophils (LDNs) are recently identified subset that more prone to develop NETs produce pro-inflammatory cytokines calprotectin than normal density. Moreover, LDNs show reduced phagocytotic activity towards bacteria. subclassified as mature (CD15+CD14-CD10+) immature (CD15+CD14-CD10-) based on CD10 expression, which most pro-inflammatory. have been number autoimmune diseases, including systemic lupus erythematosus, psoriasis, antineutrophil cytoplasmic antibody-associated vasculitis, rheumatoid arthritis, but not yet studied UC. Objective Examine existence UC patients their possible association severity. Methods A total 13 healthy controls 20 with were included. Their clinical was evaluated Truelove-Witts score. subtypes quantified by flow cytometry peripheral blood mononuclear cells (PBMC). Results The relative abundance significantly higher compared (1.55±1.14 vs. 0.62±0.36 respectively, P<0.05). numbers active those remission (2.67±1.36 1.09±0.55; P<0.05) (Figure 1). positively correlated index (rho=.530, fecal levels (OR=1.2, P<0.05, CI=1-1.36) illustrated Figure 2. 1 2 Conclusion elevated PBMCs associated activity.